Cross-cultural diversity analysis: traditional knowledge and uses of freshwater fish species by indigenous peoples of southern Punjab, Pakistan.

BACKGROUND: Fisheries have tremendous cultural and educational importance in human societies. The world is undergoing fast environmental and cultural changes, and local knowledge is being lost. Understanding how people interpret environmental change and develop practices in response to such change is essential to comprehend human resource use. This study was planned with the intent to document and conserve the knowledge about the uses of the freshwater fish fauna among the residents in South Punjab, Pakistan. METHODS: Semi-structured interviews and questionnaires were conducted to collect data from informers (N = 88). Principal component analysis, relative frequency citation, fidelity level, relative popularity level, rank-order priority, and similarity index were used to analyze the fish data. RESULTS: Overall, a total of 43 species of fishes were utilized in the study region, but only 26 species were utilized ethnomedicinally to treat a variety of illnesses such as asthma, body weakness, burn, chicken pox, cold, cough, eyesight, hepatitis, impotence, joint pain, night blindness, skin burn, spleen treatment, stomach infection, and weakness. The uses of fishes were analyzed employing various indices. The highest use value (UV) of 0.86 was calculated for spotted snakehead (Channa punctata), whereas the lowest UV of 0.05 was attained by karail fish (Securicula gora). Moreover, Channa punctata, Cyprinus carpio, Labeo rohita, Oreochromis niloticus, Wallago attu, Hypophthalmichthys molitrix, Rita rita, Sperata seenghala, Notopterus notopterus, Labeo dyocheilus, Systomus sarana, Puntius punjabensis, Securicula gora, Ompok bimaculatus, and Ompok pabda were the most popular species with RPL = 1.0. Out of the total, 20 species had a "zero" similarity index, while the ethnomedicinal use of 12 species (i.e., Labeo dyocheilus, Labeo boggut, Systomus sarana, Puntius punjabensis, Aspidoparia morar, Securicula gora, Crossocheilus diplochilus, Mastacembelus armatus, Ompok bimaculatus, Ompok pabda, Labeo gonius, and Sperata seenghala) was documented for the first time for a variety of diseases (i.e., body weakness, stomach infection, skin burn, joint pain, impotence, asthma, spleen treatment, and chicken pox). CONCLUSION: Our findings showed that the local people of the study area hold noteworthy traditional knowledge about the medicinal and cultural uses of fish species. Furthermore, a comprehensive analysis of active chemicals and in vivo and/or in vitro activities of chemicals derived from ichthyofauna with the highest FC as well as UVs could be interesting for research on new drugs.

Tooth marker of ecological abnormality: The interpretation of stress in extinct mega herbivores (proboscideans) of the Siwaliks of Pakistan.

Climate affects living ecosystems and defines species physiology. Climate change causes certain stress on animals, recorded as Enamel Hypoplasia (EH). Proboscideans, the mega herbivores, were extensively represented in the Siwaliks of Pakistan between the Middle Miocene to Pleistocene (~15.99-~0.6 Ma). This study was carried out on 15 species from 9 genera and 4 families using 319 teeth from 266 individual quarries. Our results revealed that 20.06% (64/319) of teeth were infected by EH. Family Deinotheriidae faced higher stress during the terminal of the Middle Miocene (EH 25%). Dental characters of deinotheres indicated that this family preferred soft vegetation like C3 plants and failed to survive in grassland ecology at the onset of the Late Miocene (~10-9 Ma). Gomphotheriidae (EH 21.05%) and Stegodontidae (EH 23.40%) survived through warm and dry climatic conditions of the Late Miocene, but could not survive the cool and dry climate of Plio-Pleistocene where grasslands were abundant with less browsing activity. Family Elephantidae (EH 8.47%) was successful in drier conditions and utilized the exclusive C4 diet in open grasslands as efficient grazers, indicated by their tooth morphology. Elephantids were dominant of the proboscideans in open grassland and drier climate during Plio-Pleistocene in the Indian subcontinent. We assume that change in the Siwalik palaeoenvironment was governed by a microclimate.